Clumped isotope signatures of nitrous oxide formed by bacterial denitrification
نویسندگان
چکیده
Multiply substituted isotopic species of nitrous oxide (N2O), referred to as clumped isotopes, represent a promising new tool for distinguishing production pathways this potent greenhouse gas. This work presents the first determination enrichment factors N2O isotopes during bacterial denitrification. Samples obtained after 1-, 3-, and 7-day incubations pure culture denitrifier Pseudomonas aureofaciens at 20 °C 30 were analysed by recently developed quantum cascade laser absorption spectroscopy (QCLAS) method. Enrichment εp/s cumulative product (p) relative substrate (s) determined using Rayleigh model seven most abundant isotopically molecules (isotopocules) N2O. Values (with uncertainty expressed expanded standard 95% confidence interval) two incubation temperatures (20 °C/30 °C) are: 14N15N16O (456): ε456 = (−40.3 ± 2.6)‰/(−35.1 0.7)‰ 15N14N16O (546): ε546 (−38.1 3.4)‰/(−31.2 0.6)‰ 14N14N17O (447): ε447 (21.3 1.2)‰/(24.5 0.5)‰ 14N14N18O (448): ε448 (38.8 1.5)‰/(46.4 1.2)‰ 14N15N18O (458): ε458 (−8.9 2.0)‰/(−11.7 15N14N18O (548): ε548 (−3.4 1.1)‰/(−1.8 15N15N16O (556): ε556 (−85.9 1.5)‰/(−63.9 1.4)‰
منابع مشابه
Statistical clumped isotope signatures
High precision measurements of molecules containing more than one heavy isotope may provide novel constraints on element cycles in nature. These so-called clumped isotope signatures are reported relative to the random (stochastic) distribution of heavy isotopes over all available isotopocules of a molecule, which is the conventional reference. When multiple indistinguishable atoms of the same e...
متن کاملNitrous Oxide Nitrification and Denitrification
The isotopic signatures of N and O in N2O emitted from tropical soils vary both spatially and temporally, leading to large uncertainty in the overall tropical source signature and thereby limiting the utility of isotopes in constraining the global N2O budget. Determining the reasons for spatial and temporal variations in isotope signatures requires that we know the isotope enrichment factors fo...
متن کاملKinetic explanation for accumulation of nitrite, nitric oxide, and nitrous oxide during bacterial denitrification.
The kinetics of denitrification and the causes of nitrite and nitrous oxide accumulation were examined in resting cell suspensions of three denitrifiers. An Alcaligenes species and a Pseudomonas fluorescens isolate characteristically accumulated nitrite when reducing nitrate; a Flavobacterium isolate did not. Nitrate did not inhibit nitrite reduction in cultures grown with tungstate to prevent ...
متن کاملProduction of nitric oxide and nitrous oxide during denitrification by Corynebacterium nephridii.
Resting cells of Corynebacterium nephridii reduce nitrate, nitrite, and nitric oxide to nitrous oxide under anaerobic conditions. Nitrous oxide production from nitrite was optimal from pH 7.0 to 7.4. The stoichiometry of nitrous oxide production from nitrite was 99% of the theoretical-two moles of nitrite was used for each mole of nitrous oxide detected. Hydroxylamine increases gas evolution fr...
متن کاملSubstrate oxidation and nitrous oxide utilization in denitrification.
It is generally assumed that during denitrification bacteria oxidize organic substances completely to carbon dioxide concomitant with the reduction of nitrate or nitrite to nitrogen and nitrous oxide. This assumption is based upon the early experiments of Gayon and Dupetit (1886) which demonstrated that the ratio of carbon dioxide to nitrogen in denitrifying cultures corresponded to the theoret...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Geochimica et Cosmochimica Acta
سال: 2022
ISSN: ['1872-9533', '0016-7037']
DOI: https://doi.org/10.1016/j.gca.2022.05.006